Yes, several characters die in the 2025 anime Gachiakuta, with their deaths playing significant roles in the story's emotional and plot development.

Regto is one of the most notable characters who dies. He is Rudo Surebrec's adoptive father and a Sphereite who showed compassion in a harsh world. Regto's death is a pivotal event early in the series and manga, deeply affecting Rudo's motivations and the narrative's direction. He is brutally murdered, but the exact circumstances and the killer's identity remain a central mystery explored throughout the series. His death symbolizes the loss of hope and kindness in the cruel world of Gachiakuta and serves as a catalyst for Rudo's journey.

Other deaths include various characters and entities such as members of the Raiders and Trash Beasts, which are detailed in the manga and hinted at in the anime. For example, characters like Enjin, Riyo Reaper, Jabber Wonger, and Zanka Nijiku are involved in violent confrontations that lead to deaths, as explained in the timeline of killings in the manga after season 1. The video timeline from July 2025 outlines who kills whom, indicating a sequence of deaths from earlier to later chapters, including potential deaths in season 2 and beyond.

The deaths often occur in brutal fights or as consequences of the harsh societal conditions in the Gachiakuta world, where people are treated as "trash." These deaths are not only physical but also symbolic, reflecting the characters' struggles, betrayals, and the dark environment they inhabit.

In summary, the key confirmed death is Regto's, whose murder is central to the story's emotional core and mystery. Other characters die throughout the series, mostly in violent conflicts, but Regto's death is the most detailed and significant in terms of narrative impact.