In Episode 1 'Shake Your Tush', notable comedic moments include Richard Ayoade going on the offensive with his humor, Joe Lycett delivering unusual compliments, and Daisy May Cooper revealing she has a crush on someone, which adds a personal comedic touch. There is also a segment where Joe Lycett and Lou Sanders engage in a 'You Laugh You Lose' challenge, creating a direct comedic face-off. Additionally, Daisy May Cooper performs a re-enactment of British roller coasters, such as the Dragon Ride at Legoland or Nemesis at Alton Towers, which adds physical comedy to the episode.

The first episode features ten of the UK's top comedians, including Richard Ayoade, Joe Lycett, Daisy May Cooper, Lou Sanders, Bob Mortimer, Joe Wilkinson, and Rob Beckett. Key interactions include Richard Ayoade's offensive comedic style, Joe Lycett's unusual compliments, and Lou Sanders' role as a 'starter' who provokes laughter. Bob Mortimer is noted for nervously laughing about his teeth, and there are moments of awkwardness when jokes do not land, highlighting the tension and dynamics among the comedians as they try not to laugh while making others laugh.

The format involves ten comedians competing to make each other laugh while themselves trying to keep a straight face. Any visible reaction such as laughing or smirking results in a warning, and a second offense leads to elimination. The last comedian remaining without laughing wins the LOL UK Trophy. This format is clearly demonstrated in the first episode, where comedians engage in various comedic tactics and challenges to break each other's composure while maintaining their own.

Yes, contestants use a variety of comedic styles and strategies in Episode 1. Richard Ayoade uses an offensive or aggressive comedic approach, Joe Lycett employs unusual compliments, and Daisy May Cooper uses personal storytelling and physical comedy through her roller coaster re-enactment. Lou Sanders is described as a 'starter' who provokes laughter, indicating a provocative or instigative style. The 'You Laugh You Lose' challenge between Joe Lycett and Lou Sanders also showcases direct comedic competition as a strategy.

Memorable moments include Bob Mortimer nervously laughing about his teeth, which shows the difficulty of maintaining composure. There are also awkward moments when jokes fail to get a response, such as when a comedian wiggles their 'tush' and no one laughs, creating an uncomfortable atmosphere. Lou Sanders is noted as a 'nightmare' starter, implying she frequently provokes tension. The episode captures the struggle and awkwardness of trying to be funny while not laughing, which is central to the show's tension and humor.