The four women in After 30 face various personal challenges including Temi's complicated love triangle involving Ayo and Kunle, Nkem's struggle with her desire for motherhood amidst her successful career, Aisha's silent suffocation under societal expectations to have children, and Ama's exploration of new freedoms and love after inheriting wealth, complicated further by the arrival of a new character, Alice.

After 30 explores societal pressure by showing how the women navigate expectations to settle down and have children, particularly highlighting Aisha's battle with her husband's insistence on having children and the broader societal norms that define women by their marital and maternal status.

Friendship and sisterhood are central to After 30, as the four women support each other through their personal and professional struggles, with their bond providing emotional strength despite the complexities and conflicts that arise, such as clashes over motherhood and romantic entanglements.

Romantic relationships in After 30 are portrayed as complex and sometimes challenging, including Temi's ongoing feelings for Ayo despite his involvement with another woman, the introduction of new romantic interests like Kunle and a billionaire's son, and the impact of past relationships resurfacing to complicate the present.

After 30 introduces new characters such as Kunle, who becomes part of Temi's love triangle, a billionaire's son engaged to Tam, and Alice, who adds complexity to Ama's storyline by influencing her exploration of love and freedom. These new characters bring fresh dynamics and tensions that drive the narrative forward.