The bunnies in Super Duper Bunny League originally were ordinary bunnies who gained their superpowers when Dr. Fuzzleglove used a grilled cheese maker set to the 'Super Duper' setting to create these bunny superheroes.

The main characters are the superhero bunnies, including Handsome Steve, who is noted for being super strong and funny. The team also includes a shadow-powered bunny, which is a unique power among them. The bunnies are characterized by their cuteness, adorable designs, and strong teamwork and friendship.

The villains in Season 1 include characters who cause trouble in Important City and are sent to Happy Camp to learn their lesson. Examples of villainous threats include a supervillain with a dragon that has sour breath and another who freezes people in chocolate. These villains provide mild action and danger but no immediate life-threatening situations.

The show emphasizes themes of teamwork, friendship, kindness, and optimism. The bunnies always look out for each other and prioritize working together to save the day, making the story heartwarming and wholesome despite the presence of villains and mild peril.

The storytelling is described as adorable, fun, and wholesome with a cutesy 2D animation style. The writing is top-tier with compelling stories and great character development. The tone balances mild action and danger with humor and heartwarming moments, making it appealing to both kids and adults.