In 'Clock-a-Doodle-Doo!', the main characters have distinct superpowers: Handsome Steve has super strength and the ability to fly; Midnight Bun Bun has shadow powers; Bex controls electricity; Derek can grow in size; and Stumpy has stretching abilities. These powers are central to their adventures and battles in Important City.

Annabelle, the intelligent bunny, plays a key role in 'Clock-a-Doodle-Doo!' by saving Easter from a villain who has a chip on his shoulder. She also interacts with the other bunnies and is involved in the efforts to stop the chaos caused by Bex's alarm clock turning into a roving rooster.

In the episode, Bex zaps her new alarm clock, which unexpectedly transforms into a roving rooster that threatens to destroy the city. This creates a significant challenge for the Super Duper Bunny League to resolve.

The villain in this episode is described as having a chip on his shoulder, which motivates his antagonistic actions. While specific details about his identity are limited, his conflict with Annabelle and the threat posed by the transformed alarm clock are central to the episode's plot.

The episode showcases the teamwork and friendship among the Super Duper Bunny League members as they confront the chaos caused by the roving rooster and the villain threatening Easter. Each bunny uses their unique superpowers collaboratively to protect Important City and resolve the crisis.