In The Chosen: Last Supper – Part Two, Judas shows his true colors for the first time during a tense moment after Jesus's cleansing of the Temple, where the disciples try to understand Jesus's aggression and Judas becomes more vocal about his doubts and demands that Jesus reclaim His birthright.

Jesus publicly clashes with the clergymen in the marketplace, calling them hypocrites and refusing to admit He is above the law. This confrontation ends with the clergymen leaving in anger and plotting to arrest Jesus at night, marking His last public clash with religious leaders before His arrest.

Tamar, an African woman and devoted follower of Jesus, encounters a fellow African man selling doves in the Temple courtyard. He flirts with her and ultimately gives her sacrificial doves for free, highlighting her character and cultural background within the narrative.

Part Three uses flashbacks to show critical moments that set the disciples on their path to following Jesus, including Simon the Zealot's sacrificial courage as he fights Roman soldiers alone to allow others to escape. These flashbacks reveal their old ambitions and the sacrifices they made to follow Jesus.

The movie shows Jesus grappling with the knowledge of His fate, including a spiritual conversation with Matthew about His upcoming last sermon. Jesus is depicted with kindness and grace toward His disciples while carrying the weight of the world's sin, preparing for His betrayal and crucifixion in a profoundly moving way.